Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFILGITGGIGSGKSAATQWFESQGIQVVDADIVAREVVEKGQPALQKIQQTFGDWVLQPDGSLDRRALREYIFQNPQARHTLEQIAHPAIRQSIIQQLQNPKSPYVILVSPLLFETNQHELVNHTLLIDASEQTQIQRASQRDGQNQEQIQKIIAAQMPRERKRELANDIVFNDGLLEHLYQQLEPLHQSYLKRAN 1VHT Chain:B ((5-200)) --YIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGAPALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQATREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLAS

General information: TITO was launched using: RESULT: Template: 1VHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 830 -112104 -135.06 -571.96 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -135.06 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.711

(partial model without unconserved sides chains): PDB file : Tito_1VHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHT-query.scw PDB file : Tito_Scwrl_1VHT.pdb: