Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNI-------SYELEKKIVNLNALYNEEEAEL-------SLRLSQNIR---KNQQDGYVYSLISRGFEIKGLKLWLVDKNIDQLKQWFYVSSVLRAESCKYSSGYTLSTPDEFIFPL---LSDNTEIINKFAYLDTVNLLWGEFEPSYQE--------------AKFKPSKDDPRFRTHALQAVL-----------RNDWEDYK-------------KIKEVANQKINKLR--------EVVQFEFGIYDAIYE--KDKDKIIEIVQTLL------NPRVHKTYNKDFGEEF---------NGEIWSHHPVM-FTKLAWMNGLEIEIDNPLVP---MELMPIKPLEHYDYHYDFLDPNWKPKSLFEKLFGKKRNK------ 4J3R Chain:A ((4-383)) ATLPTTASSSTAVASSQLDQLANFAYNVTTDSVAGCTLQNLRVRRDWRAFSKTQKKDYINSVLCLQKLPSRTPAHLA-PGARTRYDDFVATHINQTQIIHYT-GTFLAWHRYFIYEFEQALRDECSYTGDYPYWN-----WGADADNMEKSQVFDGSETSMSGNGEYIPNQGDIKLLLGNYPAIDLPPGSGGGCVTSGPFKDYKLNLGPAALSLPGGNMTAAANPLTYNPRCMKRSLTTEILQRYNT-FPKIVELILDSDDIWDFQMTMQGVPGSGSIGVHGGGHYSMGGDPGRDVYVSPGDTAFWLHHGMIDRVWWIWQNLDLRKRQNAISGTGTFMNNPASPNTTLDTVIDLGYANGGPIA-MRDLMSTTAGPFCYVYL

General information: TITO was launched using: RESULT: Template: 4J3R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1191 67128 56.36 240.60 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 56.36 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_4J3R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J3R-query.scw PDB file : Tito_Scwrl_4J3R.pdb: