Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLMVAVFAGMVPLASFSAQAATPEQECQKLQDDYNLIYASKGFCFKDQDAKEKYGNENCHTTKPKFSDKEQQRLDA---IKERQKELKCK 2MC5 Chain:A ((1-73)) MNEFT------QISGYVNAFGSQRGSVLTVKVENDEGWTLVEEDF------DRADYGS------DPEFVAEVSSYLKRNGGIKDLTKVLTR-

General information: TITO was launched using: RESULT: Template: 2MC5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 280 13230 47.25 189.00 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 47.25 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_2MC5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MC5-query.scw PDB file : Tito_Scwrl_2MC5.pdb: