Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEGVGLPLWLLAELTYRCLLQCPYCSNPLDYAQHKNELTTQEWFDVFDQARQ----------MGAVQLGFSG-GEPLVRQD-LEQLVA-HAHQLG-----FYTNLVTSGMGLTEQRISHLKQ-AGLDHIQISFQASDPVLNDALAGSK--HAFEQKYEMCRLVKKY----DYPMVLNFVIHRH-NI--DQIDKIIELCLELNADTVELAICQFYGWAFLNRQGLLPTQEQLIRAERITNEYREKLKAQNHPCKLIFVVPDYYEERPKACMNGWGKIFFTVAPDGMALPCHAARQLPISFPNVREQSLSRIWYESTGFNRFRGDAWMPEGCRSCPDKDRDFGGCRCQAYMLTGDASNADPVCGKSPYHQFIEQARAESEIDSSLEKLVFRNSRNSKQFTVQQNIPVQNIVDD 3RFA Chain:A ((115-349)) --------ATLCVSSQVGCALECKFCSTAQQGF--NRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALD---KLGDMIDV-ALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPC-KINLIPWNPFPGAPY----GRSSNSRIDRFSKV-------LMSYG--FTT--IVRKT---RGD------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3RFA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1027 -32420 -31.57 -156.62 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.57 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3RFA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFA-query.scw PDB file : Tito_Scwrl_3RFA.pdb: