Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFFVLSCTDN-E--GTLEKRLAVRPQHIERLQKLDDEGRLVAAGAMPKDPNDPQVGFYGSTMIVEFDTREALDEWLKEEPFLKEGIYSHVDVKPFNKAFPKG 3ZNU Chain:G ((1-89)) -MLYLVRMTVNLPRNLDPREEERLKASEKARSRTLQEQGQWRYLWRTTG--------KYGNISVFDVNSHDELHEILWSLPFFPY--L-TIDVEPLSHHPA--

General information: TITO was launched using: RESULT: Template: 3ZNU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 289 -4528 -15.67 -52.65 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain G : 0.72 3D Compatibility (PKB) : -15.67 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_3ZNU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZNU-query.scw PDB file : Tito_Scwrl_3ZNU.pdb: