Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLIASLIAISSSSIFAADTTASAAKNDVFKQAEQLYAAKNYPAAFQEVQRLAQTGN--AQAIYNLGYMTQMGQGTAKDNAKALKYYEDASNKGY--AQASYTLAQIYETGELGVAKDSNKFSQYIQKAAAQ--GSDDATVKIATILFAQKKPQSHQIALQKLAPLIRKGNYPAI---QVKALYDISQGVENKNPLMKRQGIEALQTIAQK--GYAPASMALATMMANGNIIPQNLPQAKQIFTELAKQNVPNARESLASVEKIIAEKNKQAAAAPTQPAPKK 5G05 Chain:H ((566-767)) --------------------------SPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVL----TEELDKALACFRNAIRVNPRHYNAWYGLGMIYYK-----QEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALK---KSEKALDTLNKAIVIDPKNPLCKFHRASVLFAN--------EKYKSALQELEELKQIVPKESLVYFLIGKVYKKLG----QTHLALMNFSWAMDLD---------------------------------

General information: TITO was launched using: RESULT: Template: 5G05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 837 510 0.61 2.67 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain H : 0.60 3D Compatibility (PKB) : 0.61 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_5G05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G05-query.scw PDB file : Tito_Scwrl_5G05.pdb: