Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLQQLAIKHPIFLAPMAGVSTPELAAEVSNQGGLGSLGLGANTPQSAREQILKTQALTENPFQVNFFCHQSTELNVEKAKQWLEYLRPHFEKFGAQPPQELHCIYPSFLDNDDFLNVVLETKPKTVSFHFGIPHPHQIKALKEAGILTMVSATNLIEAQAIEAAGIDIIIAQGIEAGGHRGIFN-QTFDGAIKTSDLVQLIVQHCTLPVVAAGGIMTGLQAKHMLGLGAAAVQLGTAFVQCQTSNASAEYRKALFSKPV--TQISASLSGRPARGILNHWHIKIDSPTRPVQPEYPYAYDLAKQLNALATKHQDYGFGAFWAGSNVAQIRELEAPDLVNQLVVEMLDNE 4QIU Chain:B ((14-354)) ------LGIQQPIIQAPMLGVSTPALAAAVSNAGGLGSIAITGSAAEKGRALIREVRGLTDKPFNVNLFCHRPGQADPARERAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPMLLEERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGGCRGVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAFVLCPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRMFFDAAAPGVPPLPDYPFVYDATKALQTAALARGNHDFAAQWAGQGAALARELPAAELLRTLVEEL----

General information: TITO was launched using: RESULT: Template: 4QIU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2113 -134342 -63.58 -397.46 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -63.58 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.601

(partial model without unconserved sides chains): PDB file : Tito_4QIU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QIU-query.scw PDB file : Tito_Scwrl_4QIU.pdb: