Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNLVVFSGNAHPQFAQKVVSHLHIPLGAASVGQFSDGEISVEITENVRGKDVFIVQPTCAPTNDNLMEILVMADALRRASAGRITAVIPYFGYARQDRRPRSARVPITAKVVADMLTTVGIDRVVMIDLHADQIQGFFDIPVDNIYGTPALLADLRQQ--QHDNLMVVSPDVGGVVRARAVAKQMGDIDLAIIDKRRQKANESQVMHLIGDVKDRDCVIVDDMVDTAGTLCKAADALKQFGARRVVAYATHPVLSGKAIENLRNSVIDELVVTDTIPLSEEALNLGKIRQVSVASMVAETIRRINNEESISAMFDSL 2HCR Chain:A ((3-313)) --NIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKS-RAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENISEWRNCTIVSPDAGGAKRVTSIADRL-NVDFALIHKER------DRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATRVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQEDKMKHCSKIQVIDISMILAEAIRRTHNGESVSYLF---

General information: TITO was launched using: RESULT: Template: 2HCR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1709 -121222 -70.93 -400.07 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -70.93 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_2HCR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HCR-query.scw PDB file : Tito_Scwrl_2HCR.pdb: