Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNMTRYVLELNKNIRTFLKMQLNPIYYNEQHIAFADNVRRFVQKEMTPFVNEWDEAETFPRELYKKAAEIGLLGLGFSEEYGGIPDADPFYSLLAGIEMAKAGS-GGVHISLMVHTIGAPPIQHFGSEELKARVLPGIISGEKISALAITEPGGGSDVAALQTKAVRDGDYYIVSGEKTFITSGIRADYYTVAVRTDPTKTGAEGISMLLIDAHSEGITKT-PLKKMGWWASDTAHLHFDQVRVPASNLLGKENAGFKVIMNNFNMERFFLGVVAYGYALVCYEEALEWVQQRKTFGKRLIDHQVVRHKLVDMATQLTSTRALLEETAWKMTQPKLQG-PELVAQISMLKNVATRTMQFCADAAVQTLGGMGFMRGTKSERIYREVKVNMIGGGAEEIMKDLISKQLGY 3P4T Chain:B ((24-401)) -------------------------IWTTAEREALRKTVRAFAEREVLPHAHEWERAGEIPRELHRKAAELGLLGAGFPEDAGGSGGDGADPVVICE-EMHYAGSPGGVYASLFTCGIAVPHMIASGDQRLIDTYVRPTLRGEKIGALAITEPGGGSDVGHLRTRADLDGDHYVINGAKTYITSGVRADYVVTAARTG--GPGAGGVSLIVVDKGTPGFEVTRKLDKMGWRSSDTAELSYTDVRVPVANLVGSENTGFAQIAAAFVAERVGLATQAYAGAQRCLDLTVEWCRNRDTFGRPLISRQAVQNTLAGMARRIDVARVYTRH----VVERQLAGETNLIAEVCFAKNTAVEAGEWVANQAVQLFGGMGYMAESEVERQYRDMRILGIGGGTTEILTSLAAKTLGF

General information: TITO was launched using: RESULT: Template: 3P4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2137 -49365 -23.10 -131.64 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -23.10 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_3P4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P4T-query.scw PDB file : Tito_Scwrl_3P4T.pdb: