Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDLYYWGTPNGHKITIALEEMGLDYTLYPINILENDQFQPDFLKISPNNKIPAIVDQDGPRGEPISVFESGAILQYLGRKTGLFYPTDEQERVEVEQWLMWQMGGLGPMLGQNHHFNRFA----PEKIPYAIDRYVNETKRLYGVLNKQLIRQKFVAGEYSIADMAILPWIL----RYEWQG-IQLEDYPYVQEYIVRLTARPAVQKALSIKVI 4NAX Chain:B ((21-229)) -LQLYSLPTPNGVKVSIMLEEIGLAYEAHKVSFDNDDQLSPEFISLSANNKIPAILDPNGPGGQPLPLFESGAILQYLAEKSGQLLSQDPAQRYQTLQWLMFQMGGIGPMFGQVGFFHFFAGKEYEDKRPR--DRYVNESKRLLGVLDRHLKGRQWMAEEYSIADIAIFPWVRNLVERYNARDLVGFDQFKEVQRVLANFLERPAVQHGLKI---

General information: TITO was launched using: RESULT: Template: 4NAX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 946 -66710 -70.52 -333.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -70.52 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_4NAX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NAX-query.scw PDB file : Tito_Scwrl_4NAX.pdb: