Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNIDLFQRNGRKEMLPTKQADLLYERTLVLLE-EWEETQQKLLNEQNEEEVCRIVASHTFAVYLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSL-MEDQ---LVIAGDPKSKLWLVREAN-----------SGVFHYTQRYFEEMNIQDKKMIVKSNDVI-VAL-LKEGIGRSILSKRSVP------ENVP-MT---PLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSEENLSW 3FZV Chain:A ((4-300)) YTLRQLKYFVTTVECGSVAEASRKLYIAQS-ISTAVKGLEESFV----------QLFLTPAGARFYRKAQELLRMAHEFEQNLA-DNDVIAGQIDIGCFETVAPLYLPGLIAGFRQAYPGVEIRIRDGEQQELVQGLTSGRFDLAFLYEHDLDSTIETEPLMPPQRPHALLPEGHRFAGQAQVSLRDLCLEPMILLDVQPSRTYFVSLFEELGLT-PNIAFSSPSIEMVRGMVGQGFGFSLLVTRPHSECTYDGKKVVMVDLAEPVSTSGLAAAWLKRAQLTKPARLFVDYCREQLGK------------

General information: TITO was launched using: RESULT: Template: 3FZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 19988 18.61 78.08 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 18.61 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_3FZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZV-query.scw PDB file : Tito_Scwrl_3FZV.pdb: