Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MSVDKKAAMKRIAELTKSESWQEDKEIV-----AEVQKLGKSMWTEKPKRRTPRK-----------IAIWHDDR-------ILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER---------- 1EM8 Chain:A ((1-147)) MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK

General information: TITO was launched using: RESULT: Template: 1EM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 412 16359 39.71 161.97 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 39.71 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.196

(partial model without unconserved sides chains): PDB file : Tito_1EM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EM8-query.scw PDB file : Tito_Scwrl_1EM8.pdb: