Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WTGKSEASNRPPPGRRPEETT-----WFTSDPSYACGVKIFRPGSEGSSGLAEITHLNFHEKIKVLKGQYECEVGPSCSTLRCTMPRGWRLDIFGKSFPTR-RTTYFAENGNPEYNTLASEANIPLYDCRVVIRFPPTGGRDTGPLAEATHFNLGEVVGVLG------GRHTCQAQGQCANVNCNVPKGYTYYSLTGGPFQTKEKSHTFQSDA 2FCB Chain:A ((6-178)) APPKAVLKLEPQWINVLQEDSVTLTCRGTHSPESD-SIQWFHNGNLIPTHTQPSYRFKANNND---SGEYTCQTGQT------SLSDPVHLTVLSEWLVLQTPHLEFQEGETIVL-RCHSWKDKPLV--KVTFFQ-------NG--KSKKFSRSDPNFSIPQANHSHSGDYHCTGNIG------------------YTLYSSKPVTITVQAPA

General information: TITO was launched using: RESULT: Template: 2FCB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 740 58126 78.55 361.03 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 78.55 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_2FCB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FCB-query.scw PDB file : Tito_Scwrl_2FCB.pdb: