Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNPRSLEEEKYDMSGARLALILCV-------T---KAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPGETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKR--KGHILELLTEVTRRMAEAELVQ----EGKARKTNPEIQSTLRKRLYLQ 1F1J Chain:A ((60-304)) ---------YQYNMNFEKLGKCIIINNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLLKKAS--EE-DHTNAACFACILLSHGEENVIYGKDGV-TPIKDLTAHFRGDRSKTLLEKPKLFFIQACRGTELDDGIQ---------------KIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILEEHGKDLEIMQILTRVNDRVARHFESQSDDPHFHEKKQIPCVVSMLTKELYFS

General information: TITO was launched using: RESULT: Template: 1F1J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1076 39426 36.64 184.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 36.64 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_1F1J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F1J-query.scw PDB file : Tito_Scwrl_1F1J.pdb: