Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLHGKRKEIYKYEAPWTVYAMNWSVR-PDKRFRLALGSFVEEYNNKVQLVGLDEESSEFICR--NTFDHPYPTTKLMWIPDTKGVYPDLLAT-SGDYLRVWRVGETETRLECLLNNNKNSDFCAPLTSFDWNEVDPYLLGTSSIDTTCTIWGLETGQVLGRVNLVSGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYE--------------------------DPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCT-------------PVARLNNHRACVNGIAWAPH--SSCHICTAADDHQALIWDIQQMPRAIEDPILAY-TAE--GEINNVQWASTQPDWIAICYNNCLEILRV 5U69 Chain:A ((14-367)) --------NSLKEDHNQPLFGVQFNWHSKEGDPLVFATVG----SNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIK--HYVG-----HGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIF--------GGVEGHRDEVLSADYDLLG---EKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWL---GDLILSKSCEN-AIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQK-MLALGNQVGKLYVWDLEVEDPHK-AKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDR

General information: TITO was launched using: RESULT: Template: 5U69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1691 -43374 -25.65 -141.74 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -25.65 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_5U69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U69-query.scw PDB file : Tito_Scwrl_5U69.pdb: