Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHA--GQSKLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINS-DLFVMAYATYVAALADASLQDNQQDAPLFAFLQKTLELMEAGL-DYQVLTNIFEIQILTRFGISLNFNECVFCHRVGQAFDFSFKYGTCLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLK-PGIKQELRQFMDQLYEEYV-GIHLKSKKFIDSLADWGQLLKEEKK 3Q8D Chain:A ((2-232)) ---EGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRSTLKGALQPFTP----LLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEYETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLG-------------YGVNFTHCAGSGEPVDDTMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLGGKPLKSRELFRQFM-----------

General information: TITO was launched using: RESULT: Template: 3Q8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1038 -40708 -39.22 -180.92 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -39.22 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_3Q8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q8D-query.scw PDB file : Tito_Scwrl_3Q8D.pdb: