Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MEMSFIAQDFEKLDIITVLEGRTQAIIRNHFLRYD----RVVRCRVKIITMDMFSPYYDLAR---------------QLFPCAK-----IVLDRFH---IVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWK----------------LIQQDSRKLS-------------DKHFYRPTFRM-HLTNKEILDKL-----LSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEK-------IVNALQLPYSNAKLEATNNLIKLIKRNAFGFR--------------NFENFKKRIFIALNIKKERTKFVLSRA---------------------- 3LDF Chain:A ((1-385)) MIKLMVGSFAEKKLKRGVQLLSSRDYPNLNLDNQVVQLYSDADIFLGTAYLSKQNKGVGWLISPKKVSLNVTYFIKLFQWSKDKRKNFAHSKLTTAYRLFNQDGDSFGGVTIDCYGDFVLFSWYNSFVYQIRDEIVAAFRQVYPNFLGAYEKIRFNVSAHLYGQEAPEQFLILENGISYNVFLNDGLMTGIFLDQRQVRNELINGSAAGKTVLNLFSYTAAFSVAAAMGGAMATTSVDLAKRSRALSLAHFEANHLDMANHQLVVMDVFDYFKYARRHHLTYDIIIIDPPSFARNKEVFSVSKDYHKLIRQGLEILSENGLIIASTNAANMTVSQFKKQIEKGFGKQKHTYLDLQQLPSDFAVNVQDESSNYLKVFTIKV

General information: TITO was launched using: RESULT: Template: 3LDF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1285 -815 -0.63 -3.20 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -0.63 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_3LDF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LDF-query.scw PDB file : Tito_Scwrl_3LDF.pdb: