Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMERNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGK--TLSDYTILSAARNAIAVNEHNAKMG----LVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLD---FLFAAGAFGLVIANNASISGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVKRNAMGASFAFIAADMA-LAGIESKIPVDEVIDAMYQVGASMPT---AFRETAEGGLATTPTGRRLQKEIFGE 5MJI Chain:A ((2-253)) ------------------ALKALILNTTLRRS-PSRSQTQGLIDKAVPLYEKE------------GIETEVVRVIDHDIEQEYWDDYDDWNAGEKARREDEWPWLLEKIREADILVIATPI--TLNMCTSAAHVILEKLNLMDELNGDTKQFPLYNKVAGLLMC------GNEDG-------AHHVAGTVLNNLGRLGYSVPPNAAAYWLGPA-----GTGPGYIEGKGDRHFHTNKLIRFMVANTSHLARMLQETPYTTDLEACAQAAREESDDVFAIRVNVNTPAIRYKRFQKLGEVKVEE

General information: TITO was launched using: RESULT: Template: 5MJI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1068 -25267 -23.66 -105.72 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -23.66 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_5MJI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MJI-query.scw PDB file : Tito_Scwrl_5MJI.pdb: