Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRKKFGKSC------------------------------------------------------------------------- 5MG8 Chain:A ((63-344)) DLDNIKRELSYYNDATKRKLDFMSSAPGWEDAYQTYQLLKEYESAFEAPAYGPIYMNLKCKEKGFAALIEGFFRTDTFRTFIMSNYNDYLKLMDLITSKTKYTPTIREFSSERKKKIEDFEPPCSREKLQSFGFDGYVIDFLEGPEVVLVALCHMLKIHQIPIAKRELPPASVNALNNFRLANGD------------PVLKTYLAGSSIHLVFRSAYGDREITRRTDPLPSRSIYFSENVEMDLVKRKEEQLNAQLSQLENLQNEERKLQEKVNEHESLLSRTNDILSTLRKER

General information: TITO was launched using: RESULT: Template: 5MG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 2060 79.21 54.20 target 2D structure prediction score : 0.16 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 79.21 2D Compatibility (Sec. Struct. Predict.) : 0.16 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.161

(partial model without unconserved sides chains): PDB file : Tito_5MG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MG8-query.scw PDB file : Tito_Scwrl_5MG8.pdb: