Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEAVHTEKQWERSATELPPFIIKRLPVRLTFDNNYFNDRYQVIPIGGYNVIIENILKD--VEVGLGVDCFDYREELEASA--NKVAFTGMIDQYFDYKHGELEYRSLRFKHEVLDEENHQGNAVVNYTDREIPYTRIIEHKKFE----YGLQPKTVITHEYPADWKRGDEPYYPINDEKNNAIFSKYHEEA---SQNDKVIFCGRLADYKY 5EQD Chain:B ((158-363)) -----YTAKQWQTDPKDLPASNITRLPVRYTFDNRYFNDTYEGLPVEGYTKWLENMAADERIEVRLDTDWFDVRDDLRAANPDAPVVYTGPLDRYFDYAEGRLGWRTLDFELEVLETGDFQGTPVMNYNDLDVPYTRIHEFRHFHPERTYPTD-KTVIMREYSRFADNDDEPYYPINTEADRAVLAAYRARAKAETASAKVLFGGRLGTYQY

General information: TITO was launched using: RESULT: Template: 5EQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 553 16082 29.08 82.47 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : 29.08 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_5EQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQD-query.scw PDB file : Tito_Scwrl_5EQD.pdb: