Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLIVTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGFEVGITSLGDPDTI----AAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAKADDLVVEGGH 4YMX Chain:B ((97-256)) --AGRVDMVIAGMTPTAERKKSVDFSDLYYDSRQVVVVKNDSPISKFDDLKVKTIAVQIGTTSEEA-AKKIPNVKLKQLNRVSDEFMDLQNGRCDAIVVEDTVAKAYLKEYK--DMKILYMDEINNVENGSAVAVAKGNKSLLDVVNEVIKELKQSGEYDKLVDK---------------------

General information: TITO was launched using: RESULT: Template: 4YMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 698 -32110 -46.00 -205.83 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -46.00 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_4YMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YMX-query.scw PDB file : Tito_Scwrl_4YMX.pdb: