Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNAATTPMSAVAISAMTK--VMQETHGNPSS-IHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEANNTTIIGYCLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQPE-NQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYAS---SMDFDSYLHGGDQEQKKRAGTENLPAIVGMVAALKENLEKQEEHFQHVQNLETAFLAELEGI-QYYLNRG-KHHLPYVLNIGFPGQKNDLLLLRL-DLAGISISTGSACTAGVVQSSHVLEAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG 3LVJ Chain:B ((24-397)) -IYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQL-EREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNRLWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLA---VSSGSAC----LEPSYVLRAL-GLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIG-

General information: TITO was launched using: RESULT: Template: 3LVJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2111 77339 36.64 214.83 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 36.64 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_3LVJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LVJ-query.scw PDB file : Tito_Scwrl_3LVJ.pdb: