Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTT--NKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI--KTKFDKIAPILQTIHTSAKELRG--------------EFAPFEEIKKYESLIEEQIPY------------ANY-ESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQ-GRLYLIDWEYSSMNDPMWDLAALFLES---------EFTSQEEETFLSHYESDQTP----VSHEKIAIYKILQDTIWSLWTVYKEEQG--EDFGDYGVNRYQRAVKGLASYGGSDEK 2QG7 Chain:E ((110-455)) -----------------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIAKKIYVFFT--NGRIEEFMDG-YALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTASCLPIFR-------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1036 19343 18.67 74.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : 18.67 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QG7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: