Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MEFLLYTISKVKLLEDILMPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLEMVEQLLDKVLLYDNSSI 1GRJ Chain:? ((2-85)) QAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKEN----AEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTK-

General information: TITO was launched using: RESULT: Template: 1GRJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 168 795 4.73 10.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 4.73 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_1GRJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GRJ-query.scw PDB file : Tito_Scwrl_1GRJ.pdb: