Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEA-IRQSAVFLLFVGDLNRAEKGARLHTDTFQ-PQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTS----WSQRLAEQFGQAEPSSTRKNLEQKKLL 5HEI Chain:E ((1-240)) MNEAIRTIQDHRSIRQYTDEAVSDEHLDTIIQSAQSAASSINGQQVTIISVQDKEKKKKLSELAGNQAWIDQAPLFLIFCADFNRAKIAAELNDAPLGVTDGLESILVGATDAGISLEAATVAAESLGLGTVPIGGIRRKPLEVIELLDLPEYVFPVSGLVVGHPSDHSAKKPRLPQAAVHHRESYNHDLKSLIQDYDAEMAEYMKKRTNGADDRNWSQTVSAIYKTIYYPEVRAMLEKQ---

General information: TITO was launched using: RESULT: Template: 5HEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 945 -27374 -28.97 -116.98 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain E : 0.80 3D Compatibility (PKB) : -28.97 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_5HEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HEI-query.scw PDB file : Tito_Scwrl_5HEI.pdb: