Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHFTFLGTSSGVPTLSRNVSGL---AVRNSKNKDWILVDAGEGTQHRIQQARLSLQNLIAICITHVHGDHCYGLVGLLASAGMNARNNPLIVIAPKEIQQWFEITAQLTDLHLPYSIKFIDVNEATRPQQLTDELFIQAHPLSHRVPSFAFSIYIKSTQKKIDIQALTQLGIPKGDIWGHLKRGYDVEFE-GRILKSQDFIKIQNQQIHAIIGGDNDRPELLADACKDAQLLIHEATYLQTVLDKVGKGPMHSSAKMVAEFAEQQSLDNLILTHFSPRHQDKTGQQAIIEEVRQFYKGHFYLANDFDEFTLNETGQLLKIE 1Y44 Chain:B ((1-307)) -MELLFLGTGAGIPAKARNVTSVALKLLEE--RRSVWLFDCGEATQHQMLHTTIKPRKIEKIFITHMHGDHVYGLPGLLGSRSFQGGEDELTVYGPKGIKAFIETSLAVTKTHLTYPLAIQEIEE-G-IVFEDDQFIVTAVSVIHGVEAFGYRVQEKDVPGSLKADVLKEMNIPPGPVYQKIKKGETVTLEDGRIINGNDFLEPPKKGRSVVFSGDTRVSDKLKELARDCDVMVHEATFAK-----------HSTTEQAAVTAKEARAKQLILTHISARYQGDAS-LELQKEAVDVFP-NSVAAYDFLEVNVPRG-------

General information: TITO was launched using: RESULT: Template: 1Y44.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1651 7512 4.55 25.64 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 4.55 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1Y44.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y44-query.scw PDB file : Tito_Scwrl_1Y44.pdb: