Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVG--KIELFVDRPE-GEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDEGHGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVGLRHYKTPEIQGDILLIHGEQDDITLLSDAIEWAKP-Q-KHPITILSGANHFFTGYL----KQLRQIITRFIIMK 2FUK Chain:A ((8-218)) ------TESAALTLDGPVGPLDVAVDLPEPDVAVQPVTAIVCHPLSTEGGSMHNKVVTMAARALRELGITVVRFNFRSVGTSAGSFDHGDGEQDDLRAVAEWVRAQRPTDTLWLAGFSFGAYVSLRAAAAL----EPQVLISIAPPAGRWDF----SDVQPPAQWLVIQGDADEIVDPQAVYDWLETLEQQPTLVRMPDTSHFFHRKLIDLRGALQHGVRRWLPA-

General information: TITO was launched using: RESULT: Template: 2FUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1184 -35222 -29.75 -174.37 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -29.75 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.631

(partial model without unconserved sides chains): PDB file : Tito_2FUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FUK-query.scw PDB file : Tito_Scwrl_2FUK.pdb: