Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFLPVILSLALITNASYANPFDPKPASGQVEIPNIGSGIGLLDQQKEKFIGEKVFREVHKQMPVIQDVWLEDQFFQVFSNILSETQLGQPIALVVIKD----PQINAFAVP----GGLFALNTGLISSARNIDEIAGVMAHEIAHVSQRHFSRSEEAFKGQTLLSLAGLLAGVALA-----AQAGG----D------AG--AAV-----MLGT-QAALLDKQLTYSRNQEREADRIGMQYMYAAGYNPQSMADYFETMHRATSR----VSFLPDFWLTHPLTSERMSEARLRANQMPKVKSRIYDVDFEILKWYTMVVAGEATENQLQSLASQKNLAGLLALSAFYLKQGDYTQAQATLEQAKSSGKPLVALIQTDIYLGQNKINQAYNSIAPLQMTMPENKAFSYKLAEVLLRQGKYAQVQTLVQRFINKNARDIQGWQLLQQAANLDKNSPLRAVNVLRYRAEAQYWSGSEEDAIKSMLHAQRLAKGNQAMSARIDSRLKQMQDERRMKI 4IL3 Chain:A ((230-445)) ------------------------------------------------------------------------GELKKSIESLADRVGFPLD-KIFVIDGSKRSSHSNAYFTGLPFTSKRIVLFDTLVNSN-STDEITAVLAHEIGHWQKNHIVNMVIFSQLHTFLIF-SLF-TSIYRNSSFYNTF--SGFVDPVITKEFPIIIGFMLFNDLLTPLECAMQFIMSLISRTHEYQADAYAKKL----GY-KQNLCRALIDLQIKNLSTMNVDPLYSSYHYSHPTLAERLTALD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4IL3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 535 -18694 -34.94 -108.06 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -34.94 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_4IL3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IL3-query.scw PDB file : Tito_Scwrl_4IL3.pdb: