Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRDTVFLKVESRLGYDFSGDIKIGGKYTSLIEHAGLVFISGQIPRVGDTVQICGKVGLDVDL-SDAQLAASIS----TMRALAILKQHYGTLDVIGKVLQMNVFVHSTSSFIQ----QSEVADGASEILYEILGLDTGRHTRTSVSVYQLPKNASVEINFIFALK 3D01 Chain:L ((10-164)) --MSDVIEGRLKELGFTLPVA----ANYVPFTISGNLLYVSGQLPMESGKIAVTGLVGRDV-DVASAQRAAELCAVNILAQVKAALNGDLSKIR---RVIKLNGFVASVPEFVEQHLVIN----GASNLIATVLG-EPGRHARAAVGMASLPFNASVEIDAIVEID

General information: TITO was launched using: RESULT: Template: 3D01.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 676 -8418 -12.45 -59.28 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain L : 0.73 3D Compatibility (PKB) : -12.45 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_3D01.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D01-query.scw PDB file : Tito_Scwrl_3D01.pdb: