Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELRHLRYFVAVVEEQSFTKAAEKLFIAQPPLSRQIQNLESELGIQLFERGSRPLQTTPAGHFFYQHAVKLLSNAEEIKSMTKRIGLIERSMTIGFVGSLLYGLLPRIIYLFRQQQPHLNIQLMELSTTEQLQALKEGRIDVGFGRLRISDPAVRRILLRKERLVVAAHTSHPIAQ-RTEGVYLADLIDEKMFMYPTSLKPNFSTQLLNIFAEHSLVPKNMHEIREIQLALGLVAAGEGICIIPASADTIRFPHLNYIPILDNGAVSPIFITARAMDRSEDLQSLFDCIYQVYDLEGIPYQRTVFTLDQNPIDDSNGIDF 2H99 Chain:B ((89-297)) ----------------------------------------------------------------------------------------EKTIRIGFVGSLLFGLLPRIIHLYRQAHPNLRIELYEMGTKAQTEALKEGRIDAGFGRLKISDPAIKHSLLRNERLMVAVHASHPLNQMKDKGVHLNDLIDEKILLYPSSPKPNFSTHVMNIFSDHGLEPTKINEVREVQLALGLVAAGEGISLVPASTQSIQLFNLSYVPLLDPDAITPIYIAVRNMEESTYIYSLYETIRQIYAYEG-----------------------

General information: TITO was launched using: RESULT: Template: 2H99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1063 -107387 -101.02 -516.28 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -101.02 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_2H99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H99-query.scw PDB file : Tito_Scwrl_2H99.pdb: