Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKALPIAVAVVLGGAALVPVYYATQHPTTEVGHKADKNASPIQKISYVLGYEVAQQTPPELDTKAFVQGIHDVRNKQPSAYTQEDLKAAVAAYEKELQQKMQHQDKPEQAGTATDSADAQFLAENKTKAG--VKTTASGLQYIITKEGTGKQPTAQSIVKVHYEGRLINGQVFDSSYKRGQPVEFPL--NQVIPGWTEGLQLMKEGGKATFFIPSNLAYGPQELPG-IPANSTLIFDVELISVK 4GGQ Chain:D ((69-209)) ----------------------------------------------------------------------------------------------------RIQADQTPED----LDMEDNDIIEAH-----STVVTTESGLKYEDLTEGSGAEARAGQTVSVHYTGWLTDGQKFDSSKDRNDPFAFVLGGGMVIKGWDEGVQGMKVGGVRRLTIPPQLGYGARGAGGVIPPNATLVFEVELLDV-

General information: TITO was launched using: RESULT: Template: 4GGQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 520 -3322 -6.39 -25.55 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -6.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_4GGQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GGQ-query.scw PDB file : Tito_Scwrl_4GGQ.pdb: