Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYCQFFSVLALGLSAASCAVTSGLQTYDIPSEGVYKTDLGTTVNVVKISQETLPAIQPAQIDYQRDYASLFKNQQTIYRLSPGDVLSIQLWAYPEIAPPVNNISNEQSVQAYGYPIDQTGYIQFPLVGRYKAAGKTLAQVNRELHSQLARFLKNPDVVVRVVSYEGQRF-SVQGSVTKGGQFYLSDQPVSIYTALGMAGGVTTT--GDNTYIQLIRNGRTYNLNTIDLEKAGYSLHKLLVQPNDTIYVSTRENQKIYVMGESGKNQALPMRDQGMSL---TDALGESLGINPLSGSASRIYVVRTNPNDRTTEIYHLNLMSIGDFGLANQFRLRSNDVVYVDATGLTRWQRIVNQIIPFSNALYNIDRLGQ 5U5G Chain:A ((3-288)) ------RVVGFIGLGRMGQAICRRLLASQMP------------VHVHNRSREKADDLIRQGAVWAPDIVALTRAARVLFVCTAG----------------------SEAVQDFYHAPDRG----------------------------LLACLEVGDIVVDLSTIAPETAEGLHAAFAQQGADYIE-CPVSGGVEGALAGILSAIVSGRPEAYGLIRPLLEVFCATVTYVPEPGKAQRLKILNNLAESINLAGAIEVISQGLSQGLDLKSMADVFTSCRGRSAYMDVALGYALSGGASSNVS---------------LGVRCK-DLELARR-RLPQDQSYPFSTLAMTTFDTVRQACGEESDQCQYFSVLSH

General information: TITO was launched using: RESULT: Template: 5U5G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1469 -101499 -69.09 -362.49 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -69.09 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.297

(partial model without unconserved sides chains): PDB file : Tito_5U5G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U5G-query.scw PDB file : Tito_Scwrl_5U5G.pdb: