Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQQYRLDELAHLVKGELIGEGSLQFSNLASLENAEVNHLTFVNGEKHLDQAKVSRAGAYIVTAALKEHLP-EKDNFIIVDNPYLAFAILTHVFDKKISSTGIESTARIHPSAVISETAYIGHYVVIGENCVVGDNTVIQSHTKLDDNVEVGKDCFIDSYVTITGSSKLRDRVRIH------------SSTVIGGEGFGFAPYQGKWHRIAQLGSVLIGNDVRIGSNCSIDRGALDNTILEDGVIIDNLVQIAHNVHIGSNTAIAAKCGIAGSTKIGKNCILAGACGVAGHLSIADNVTLTGMSMVTKNISEAGTYSSGTGLFENNHWKKTIVRLRQLADVPLTQITKRLDHIQAQIESLESTFNLRK 4IHH Chain:C ((12-344)) ------RLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVVMT---QDDLPFAKSAALVVKNPYLTYARMAQILD-----TTPQPAQNIAPSAVIDATAKLGNNVSIGAN------AVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTIYHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDD-----MSKRLKSLERKV-----------

General information: TITO was launched using: RESULT: Template: 4IHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1764 -88932 -50.41 -277.91 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -50.41 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4IHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IHH-query.scw PDB file : Tito_Scwrl_4IHH.pdb: