Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVE-KN-ILNPEAVAEALERAMNLANPQTTHAAIAVPTSTVIHKTIEMDA-DMSDDER-EVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANP-NRVNVL-LVATRTENVETRVEVLELADLNPKLADVESYAVERTFSVFADSLPIGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEEASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFLQMGFSPQ-VDVQKIENDASSLMVACGLALRSFD- 2YCH Chain:A ((11-375)) PRVEALGLEIGASALKLVEVS--GNPPALKALASRPTPPGLL--MEGMVAEPAALAQEIKELLLEARTRKRYVVTALSNLAVILRPIQVPKMP---LKEMEEAVRWEAERYIPFPI-EVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEALRGAGLVPVVLDVKPFAGLYPLEARLAEEP--DRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLAAEEVKRT----YGRIYDAIRPVLVELTQELRRSLEFFRIQLS---PEVGYLLGGGSKLRGLASLLTDTLGVNLEPVNPWEAVAVDPKRFESEQLQEIGPEFAVALGLALRGVEP

General information: TITO was launched using: RESULT: Template: 2YCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1585 18407 11.61 56.99 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 11.61 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_2YCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YCH-query.scw PDB file : Tito_Scwrl_2YCH.pdb: