Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLCFDVSNLFRSSLRVIMQHPTSTDIQRVREFLLDLQARICAGLEQQEKAGGGTAEFIIDDWERPEGGGGRSRVLQNGTVIEKGGVMFSHINISKLPPSATERHPQIAGAKAQALGVSLVIHPKNPNIPTSHANVRLFVAEREGQDPIWWFGGGFDLTPFYPDDQDVLNWHQAAYDLCKPFGDNVYAEHKKWCDDYFYLKHGDEQRGVGGLFFDDLNCWDFETCFKYIQAVGNGYLNAILPIFEKHREQPYTEAQREFQLYRRGRYVEYNLVYDRGTLFGLQTGGRIESILVSLPNLAAWSYRPEWDEDSPEKRLTDYYLKPRDWLGLEEKVA 2QT8 Chain:B ((9-309)) ----------------------MSLAVEAVKDFLLKLQDDICEALEAEDG----QATFVEDKWTREGGGGGRTRVMVDGAVIEKGGVNFSHVYGKGLPMSSTERHPDIAGCNFEAMGVSLVIHPKNPHVPTSHANVRLFVAEREGKEPVWWFGGGFDLTPYYAVEEDCRDFHQVAQDLCKPFGADVYARFKGWCDEYFFIPYRNEARGIGGLFFDDLNEWPFEKCFEFVQAVGKGYMDAYIPIVNRRKNTPYTEQQVEFQEFRRGRYAEFNLVIDRGTKFGLQSGGRTESILISLPPRARWGYNWQPEPGTPEARLTEYFLTKRQWV-------

General information: TITO was launched using: RESULT: Template: 2QT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1614 -134892 -83.58 -448.15 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -83.58 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2QT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QT8-query.scw PDB file : Tito_Scwrl_2QT8.pdb: