Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MECSMNLADRDGFIWQDGQLIDWRDAKIHVLTHTLHYSMGVFEGVRAYETPKG-TAIFRLQDHTKRLLNSAKIYQMKVPYDQAALEQAQIDVVRENKLASCYLRPLIWIGSEKLGIAATNNTIHAAVAAWAWGAYLGDEAMAKGIRVKTSSFTHHHPNVTMCKAKASGNYTLSILAHQEVAHSGYDEAMLMDPQGYVCQGSGENVFLIKDGVLHTPDIAGGALDGITRQTVMTIAKDLGYEVVERRITRDEFYIADEAFFTGTAAEVTPIREYDDRQIGAGCRGPITTEIQKTFFDAVQGKKPKYEHWLTYVK 3U0G Chain:C ((23-328)) -----SMADRDGKIWMDGKLIEWRDAKIHVLTHTLHYGMGVFEGVRAYKTADGGTAIFRLKEHTKRLLNSAKIFQMDVPFDQETLEAAQRDVVRENKLESCYLRPIIWIGSEKLGVSAKGNTIHVAIAAWPWG-------LAKGIRVKTSSFTRHHVNVSMVRAKASGWYVNSILANQEATADGYDEALLLDVDGYVSEGSGENFFLVNRGKLYTPDLA-SCLDGITRDTVITLAKEAGIEVIEKRITRDEVYTADEAFFTGTAAEVTPIRELDNRTIGGGARGPITEKLQSAFFDVVNGKSAKHADWLTKI-

General information: TITO was launched using: RESULT: Template: 3U0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1597 -17830 -11.16 -59.83 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.91 3D Compatibility (PKB) : -11.16 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_3U0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U0G-query.scw PDB file : Tito_Scwrl_3U0G.pdb: