Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILIVHAHPEPLSFTTSLKTTAQQTFKKLGHQVVISDLYAMQFNPLASKEDFL-ELNQPDYFNYALEQRNATKQQLLSPDIQAEIDKVKRADLIILNFPLYWTSVPAILKGWIDRVFVSGLFYGGKRFYNHGGMAGKKAMLCLTLGGRTHMFGENSIHGPIEHYLSPIQRGTLAYTGFEVLPPFIAYHVPYISQEARETILEDYVTYLTHLDQLEPLKFPKLEEFDEKLYPL 4ZVN Chain:B ((4-220)) -KVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPI---------------

General information: TITO was launched using: RESULT: Template: 4ZVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1030 -86343 -83.83 -399.74 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -83.83 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_4ZVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZVN-query.scw PDB file : Tito_Scwrl_4ZVN.pdb: