Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALKDRLKDSRIKAKK--TQAEVAEAVKM-SQPAYQALESGKNLKSAFLPLIAQFLGVDAYWLTTGNSEDSFRESDVFSPTVVNNDAADQYVWIEVVEASFSCGTGESIEFHFDAINGKIPFPASFFKEKRVAQECMRIIKAKGDGMTD-YIKDGDLVGIDISQTEVIDGEIYAFYFAGEGMIKQIFKEADGSLILHSLNEKFRDRRVTEENGKNFKVMGRQFWRAG 1JHH Chain:A ((12-196)) ---FDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALAR-KGVI-EIVSGASRGIRLL------------------------QEEEEGLPLVGR-VAAGEPLLAQQH---IEGHYQVDPSLF-----KPNADFLLRVSGMAMKDIGIMDGDLLAVHKTQ-DVRNGQVVVARIDDEVTVKRLKKQ-GNKVELLPENSEFKPIVVDLR-QQSFTIEGLAVGVI-

General information: TITO was launched using: RESULT: Template: 1JHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 727 33633 46.26 185.82 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 46.26 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_1JHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JHH-query.scw PDB file : Tito_Scwrl_1JHH.pdb: