Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALTSIAPVINQYGV--TVSTYSEIVEHLKEKYREIYG----QDVY---LENDSQDGQWIGVIARVVADCNAAVADVYSSMSPSTAGTDALSRNVKINGIRRAVATKSTVSVVLVGAAGTIINNGIVSDKNNNRW--LLPTQVVIPPEGEIFVAASAEKPGAIL-------------------ALPNSITTIST-PTRGWQSVNNPQASTLGAPVESNVKLR-------QRQALSTAIPS--------RSYTEGILGALFSLDGVSRCKVYENKKSFVDPLEL-PPNSLSVVVAGG-DDQLIAETIRVKKAPGCDLYGNTTVIRPTVYGDPVEIQY--WRPVQKSIGLRFELTTNSDYTVDIGEQ-------IKSATADYINQLDIGDR--IAINKLYVPAGLYGALDARSYEIESLQLTVD-GVPVEGDYTLDFNAVAYCDSDDIEISVAGGG---------- 5DIY Chain:B ((2-473)) EYFRYRGIIEGFYGKPWEHQERLDMFEFMQANNLNAYIYAPKQDLYHRELWREPYKEEQLQLFKELIEKAGSCGINFTFAISPGLSLV--YSSEEELETLIRKITPFLEMGVHSIGIFFDNVPFDLIHEEDRNSYSNLAEAQADFLTRVLQRLESTISTPQIIMCPTFYCNDPNLEYLRILGQRLPKNIDVFWTGPNVCSHEITTSHMQEVQKSLQRPATLWDNYPVNDGGMMPELHIGPYDHRDPELHTHVVGIYANPMALPEASKLPLYTFAQYLNSPSQYNPQDSWRQAVSTLLGEDNLSAMEKFYQSNTISCLEPEEPAYLTNLFKKVQEDFASFRFEQGLRTLREEIISMQTTYSRLSTQDSKFFWEIRPWLEEYKLWTDYLDQAMITFSNLFARESLQKALQGRTYLREVLKDAVDFRTRVCGDVVRNFLQQVLRSTVSIELQAEGKEWTALPPGIVR

General information: TITO was launched using: RESULT: Template: 5DIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2013 32112 15.95 81.92 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 15.95 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.131

(partial model without unconserved sides chains): PDB file : Tito_5DIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DIY-query.scw PDB file : Tito_Scwrl_5DIY.pdb: