Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLRVLVTTTALLAAGAAMADEAVVHDSYAFDKNQLIPVGARAEVGTT-GYGGA----LLWQANPYVGLALGYNGGDISWTDDVSVNGTKYD-LDMDNNNVY------LN-AEIRPWGAS--------TNPWAQGLYIAAGAAYLDNDYDLAK-RIGNGDTLSIDGKNYQQAVPGQEGGV--------------------------------RGKMSYKNDIAPYLGF------GFAPKISKNWGVFGEVGAYYTGNPKVELTQYNLASVTGNPTSAQDAVDKEANEIRNDNKYEWMPVGKVGVNFYW--------------------------------- 2HJS Chain:A ((5-338)) -QPLNVAVVG----ATGSVGEALVGLLDERDFPLHRLHLLASAESAGQRMGFAESSLRVGDVDSFDFSSVGLAFFAAAAEVSRAHAERARAAGCSVIDLSGALEPSVAPPVMVSVNAERLASQAAPFLLSSPCAVAAELCEVLAPLLATLDCRQLNLTACLSVSSLGREGVKELARQTAELLNARPLEPRLFDRQIAFNLLAQVGAVDAEGHSAIERRIFAEVQALLGERIGPLNVTCIQAPVFFGDSLSVTLQCAEPVDLAAVTRVLDATKGIEWVGEGDYPTVVGDALGQDE---TYVGRVRAGQADPCQVNLWIVSDNVRKGAALNAVLLGELLIKHYL

General information: TITO was launched using: RESULT: Template: 2HJS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1172 -5827 -4.97 -24.18 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -4.97 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.121

(partial model without unconserved sides chains): PDB file : Tito_2HJS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HJS-query.scw PDB file : Tito_Scwrl_2HJS.pdb: