Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISKDIKMLALVTGASAGFGYSISKKLIESGYNVIGCGRRAEKLEELQKQLGENFYPLVFDMTDTAENINKLFKELPNEFQIDQIDLLVNNAGLALGLEPADKADLDDWYTMIDTNVKGFVTVTRLILPSMVKKKSGLIINMGSIAGTYPYPGGNVYGATKAFVEQFSLNLRADLAGTGVRVTNIEPGLCGGTEFSLVRFKGDQEKANSLYDKKNPILPEDIANTVAWIASQPPHININRIEMMPTTQTFNPLKVVEVE 3ASV Chain:C ((1-245)) --------MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAA-IEEMLASLPAEWC--NIDILVNNAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSYAGLNV----

General information: TITO was launched using: RESULT: Template: 3ASV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1292 -128293 -99.30 -523.64 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -99.30 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_3ASV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ASV-query.scw PDB file : Tito_Scwrl_3ASV.pdb: