Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFY----LHSPFRLNITLRSKTMN-------------AKFNPVTKLVEVQKGEPTTTTLQIALGL---------------------------------------GLTHKSVI------QLVRTYLP--DIQEFGRVRFESCNSAFEMANSGFDVRNSNQGRHTRYAILNE--------------QQAYFLMTLMRNSPRVIDFKKALVKSFFEARVLLQ-TDYFALI--QQREAL--------------NAKLECEKEIASSCGKGLATWKKQRDCLTTAIANVDRQ--------IQPCLFDGETI 4IJY Chain:A ((22-326)) DIRGCPTLETPLKLTFTEDIQPRKENYFYYDGWRGVGQTVNPWSPVLDNHKYAATEHEIHIYVEFFQTPSNRFADKNGAYSYIDANGVMYTNGEYSWEHVPALGKNIYKVVISDWNKGQTKSIYLPGRDFKTVEVFHFQNNRPQWDDRNSYENVKSRINNNISKSYSKAKLNEQLSTYVHDDGTDSLFLYQKLSRASLKESQINYYQLRGKFNGVNLGYWAQEYILFGGEGAEQLKNKIPDMSNYSMEDNGSFKNALKIESLDLRLMDNNRMAYGSTGTYIASFNRTDFSMTPENLKACGLD----

General information: TITO was launched using: RESULT: Template: 4IJY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 786 15359 19.54 77.18 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 19.54 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.091

(partial model without unconserved sides chains): PDB file : Tito_4IJY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IJY-query.scw PDB file : Tito_Scwrl_4IJY.pdb: