Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNHFATELDELKQQGNFRQF-TQNVQHGRFITIQNKTMLNLASNDYLGLAADINLRQEFLDSYPLERSYFSSSSSRLLTGNFDEYEQLENSLSQAFSGRAALLFNSGYHMNIGILPAVADSKTLILADKLVHASMIDGIRLSGAQYVRYRHNDLQHLEQLLQKYHDDDQIERIIVVTESIFSMDGDETDLTALVQLKKRFAKTMLYVDEAHSIGVRGQQGLGCAEQYNVIQEIDFLVGTLGKALAAVGGYIICDQIIKDYLINKMRPLIFSTAQPPIVMAWANFIFKKV--LMAQSQREHLKNISQYLQQAVVQKGYV-SPSTSHIIPVIVGESQATIDKAKQVQESGFYAMPVRPPTVPKNSSRLRISLTSMVEQHELEALVSFL---- 1BS0 Chain:A ((2-384)) -SWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNFSSNDYLGLSHHPQIIRAWQQGAEQFGI--GSGGSGHVSGYSVVHQALEEELAEWLGYSRALLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLARLLASPCP----GQQMVVTEGVFSMDGDSAPLAEIQQVTQQH-NGWLMVDDAHGTGVIGEQGRGSCWLQKV--KPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQLAEKLRQQGCWVTAIRPPTVPAGTARL-LTLTAAHEMQDIDRLLEVLHGNG

General information: TITO was launched using: RESULT: Template: 1BS0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2113 -70135 -33.19 -187.53 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -33.19 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_1BS0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BS0-query.scw PDB file : Tito_Scwrl_1BS0.pdb: