Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFVVTENCIKCKYQDCVEVCPVDCFYEGPNFLVINPDECIDCALCEPECPANAIFSEDELPEGQEVFIELNAELSQKWPNITQIGEQPADREEWNGKPDKLQYLEK 1GAO Chain:C ((1-106)) -AFVVTDNCIKCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCASCEPECPAQAIFSEDEVPEDMQEFIQLNAELAEVWPNITEKKDPLPDAEDWDGVKGKLQHLER

General information: TITO was launched using: RESULT: Template: 1GAO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 526 -70502 -134.03 -665.11 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.89 3D Compatibility (PKB) : -134.03 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_1GAO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GAO-query.scw PDB file : Tito_Scwrl_1GAO.pdb: