Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MRFK-------DMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNILDEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPRTIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYTDLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWL--LSPNLTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQLLAG-EHIGSGLGILTS------ 5CZL Chain:A ((23-337)) LPRAWADTAWESYKSRFMMADGRIVDTGNGSVSHTEGQGFAMLLAVAKNDRPAFDKLWQWTDKTLRNKDNGLFYWRYNPVAPDPIADKNDA---TDGDTLIAWALLRAQQQWGDKSYGIASDAITASLLKSTVITFAGHQVMLPGAKGFNRNDHVNLNPSYFIF-PAWQAFAARTHLTAWRKLQSDGKALLGKMAWGKAQLPSDWVALRADGRMEPAKEWPPRMSYDAIRIPLYLSWADPQSALLTPWKSWFQSYPRLQTPAWVNVNTNDVAPWFMTGGLLAVRDLTTGEA---QDDPQLSAQDDYYSASLKMLVWLAKNDR

General information: TITO was launched using: RESULT: Template: 5CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1476 20261 13.73 71.09 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 13.73 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.168

(partial model without unconserved sides chains): PDB file : Tito_5CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CZL-query.scw PDB file : Tito_Scwrl_5CZL.pdb: