Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKTIILGGFTEMKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAALITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDG-FQY--FQSS-NESDYIPNIDQALNAGFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVAAAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDKGRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYTLNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTE-GQLSDEAKKAVDEAKEKIISGDVKVPETPEEN 3S99 Chain:A ((26-319)) ----------------------------------------------------KLKVGFIY-IGPPGDFGWTYQHDQARKELVEAL------GDKVETTFLENVAEGADAERSIKRIARAGNKLIFTTSFGYMDPTVKVAKKFPDVKFEHATGYKT-ADNMSAYNARFYEGRYVQGVIAAKMSKKGIAGYIGSVPVPEVVQGINSFMLGAQSV-N---PDFRVKVIWVNSWFDPGKEADAAKALIDQGVDIITQHTD--STAAIQVAHDR------GIKAFGQASDMI--------KFAPDTQLTAVVDEWGPYYIDRAKAVLDGTWKSQNI-WWGMKEGLVKMAPFTNMPDDVKKLAEETEARIKSGELNPFTGP---

General information: TITO was launched using: RESULT: Template: 3S99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1750 46834 26.76 162.05 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 26.76 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_3S99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3S99-query.scw PDB file : Tito_Scwrl_3S99.pdb: