Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKDNITEILR---KENYSRGVVVRQEDNGIAVDVYTIISYGTKI-------SEVSRNVQEKVKYNLEA-LLGVTANSVNVFVQGVRVLPD---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 5UFL Chain:A ((5-561)) TACLKSLLLTVSQYKANATQLLRHLEVISRLFTSNQILTSECLSCLVELLEDPSASLILSIIGLLSQLAVDIETRDCLQNTLNSVLAGVVCRTDSVFLQCIQLLQKLTYNVKIFYSGANIDELITFLIDHIQSELKMPCLGLLANLCRHNLSVQTHIKTLSNSFYRTLITLLAHSSLTVVVFALSILSSLTLNEEVGEKLFHARNIHQTFQLIFNILINGDGTLTRKYSVDLLMDLLKNPKIADYLTRYEHFSSCLHQVLGLLNGKDPDSSSKVLELLLAFCSVTQLRHMLTQMMFESHTKCLEPTVALLRWLSQPLDGNCSVLALELFKEIFEDVIDAANCSSADRFVTLLLPTILDQLQFTEQNLDEALTRKKCERIAKAIEVLLTLCGDDTLKMHIAKILTTVKCTTLIEQQFTYGKIDLGFGTKVADSELCKLAADVILKTLDLINKLKPLVPGMEVSFYKILQDPRLITPLAFALTSDNREQVQSGLRILLEAAPLPDFPALVLGESIAANNAYRQQE

General information: TITO was launched using: RESULT: Template: 5UFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 386 -15320 -39.69 -127.67 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -39.69 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.147

(partial model without unconserved sides chains): PDB file : Tito_5UFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UFL-query.scw PDB file : Tito_Scwrl_5UFL.pdb: