Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTATFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPT-GGYL----IGFLLQTWITGWMIERTDAHYLYAIFAN-LMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATGAAYIGILMKKRLNRFLHTN 5D3M Chain:G ((9-173)) SKLELRELVLLAMVIAIKVILGQFKVGNATLQVGLGFIGSVMLGYLFGPWWGFAGGALSDLV---------------SSVIFGNLGGFFIGFTLTAALGPMIYGFFLYKQPIQIWRVIASVICVTVICNIGLNTLWVSMMYGINFMVALSSRILKEMITPWIQMVAVWFILEGLSRVKLS-----

General information: TITO was launched using: RESULT: Template: 5D3M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 623 -99086 -159.05 -623.18 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain G : 0.67 3D Compatibility (PKB) : -159.05 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_5D3M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D3M-query.scw PDB file : Tito_Scwrl_5D3M.pdb: