Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRYTGPSWKISRRLGISLSGTGKE-------LARRPYAPGQHGPNSRGKQSEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRLGLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATVGRPA--FVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL 5BR8 Chain:D ((2-209)) -GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRPSDYAVRLREKQKLRRIYGISERQFRNLFEEASK-KKGVTGSVFLGLLESRLDNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQNLEAMKGRKVGPWLSLDVEGMKGKFLRLPDREDLALPVNEQLVIEFYSR--

General information: TITO was launched using: RESULT: Template: 5BR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 867 33723 38.90 169.46 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.84 3D Compatibility (PKB) : 38.90 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_5BR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BR8-query.scw PDB file : Tito_Scwrl_5BR8.pdb: