Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGKVSDIEGLIEKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYSLRLAQEISYIYGFKDLMGENGELSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLSMKPMATKLQTELGKNVAYTEESLQQDLDILEGEFEEVSSDIASSDTVLSQLERLSHLLETNMITEEEFQQLKQELLKK 3A1G Chain:A ((725-748)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DARIDFESGRIKKEEFTEIMKICS--

General information: TITO was launched using: RESULT: Template: 3A1G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 33 -4921 -149.11 -205.02 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.48 3D Compatibility (PKB) : -149.11 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 1.129

(partial model without unconserved sides chains): PDB file : Tito_3A1G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3A1G-query.scw PDB file : Tito_Scwrl_3A1G.pdb: